› 1noh

X-ray diffraction
2.8Å resolution

The structure of bacteriophage phi29 scaffolding protein gp7 after prohead assembly

Released:
DOI: 10.2210/pdb1noh/pdb
PDB entry 1noh coloured by chain, front view.
PDB entry 1noh coloured by chain, side view.
PDB entry 1noh coloured by chain, top view.
Source organism: Bacillus virus phi29
Primary publication:
Bacteriophage phi29 scaffolding protein gp7 before and after prohead assembly.
Morais MC, Kanamaru S, Badasso MO, Koti JS, Owen BA, McMurray CT, Anderson DL, Rossmann MG
Nat Struct Biol 10 572-6 (2003)
PMID: 12778115

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-146795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid assembly scaffolding protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 97 amino acids
Theoretical weight: 11.17 KDa
Source organism: Bacillus virus phi29
Expression system: Escherichia coli
UniProt:
  • Canonical: P13848 (Residues: 2-98; Coverage: 99%)
Gene name: 7
Sequence domains: Phi29 scaffolding protein
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2221
Unit cell:
a: 114.005Å b: 117.301Å c: 105.933Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.254 0.29
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Virus maturation: dynamics and mechanism of a stabilizing structural transition that leads to infectivity.
Steven et al. (2005)
13 more

5 mentions without citation

Virus maturation.
Veesler et al. (2012)
4 more