› 1noy

X-ray diffraction
2.2Å resolution

DNA POLYMERASE (E.C.2.7.7.7)/DNA COMPLEX

Released:
DOI: 10.2210/pdb1noy/pdb
PDB entry 1noy coloured by chain, front view.
PDB entry 1noy coloured by chain, side view.
PDB entry 1noy coloured by chain, top view.
Source organism: Tequatrovirus T4
Primary publication:
Crystal structures of an NH2-terminal fragment of T4 DNA polymerase and its complexes with single-stranded DNA and with divalent metal ions.
Wang J, Yu P, Lin TC, Konigsberg WH, Steitz TA
Biochemistry 35 8110-9 (1996)
PMID: 8679562

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114273 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA-directed DNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 45.32 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04415 (Residues: 1-388; Coverage: 43%)
Gene name: 43
Sequence domains: DNA polymerase family B, exonuclease domain
Structure domains:
DNA (5'-D(*TP*TP*T)-3') Chain: S
Molecule details ›
Chain: S
Length: 3 nucleotides
Theoretical weight: 868 Da
Source organism: Tequatrovirus T4
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 114.701Å b: 109.656Å c: 70.268Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

Structural and functional insights provided by crystal structures of DNA polymerases and their substrate complexes.
Brautigam et al. (1998)
7 more

15 mentions without citation

Bacteriophage T4 genome.
Miller et al. (2003)
14 more