› 1noz

X-ray diffraction
2.2Å resolution

T4 DNA POLYMERASE FRAGMENT (RESIDUES 1-388) AT 110K

Released:
DOI: 10.2210/pdb1noz/pdb
PDB entry 1noz coloured by chain, front view.
PDB entry 1noz coloured by chain, side view.
PDB entry 1noz coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Crystal structures of an NH2-terminal fragment of T4 DNA polymerase and its complexes with single-stranded DNA and with divalent metal ions.
Wang J, Yu P, Lin TC, Konigsberg WH, Steitz TA
Biochemistry 35 8110-9 (1996)
PMID: 8679562

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-137632 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed DNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 45.32 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04415 (Residues: 1-388; Coverage: 43%)
Gene name: 43
Sequence domains: DNA polymerase family B, exonuclease domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 113.617Å b: 109.309Å c: 68.577Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 not available
Expression system: Escherichia coli

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Citations

9 review citations

Bacteriophage T4 genome.
Miller et al. (2003)
8 more