› 1np8

X-ray diffraction
2Å resolution

18-k C-terminally trunucated small subunit of calpain

Released:
DOI: 10.2210/pdb1np8/pdb
PDB entry 1np8 coloured by chain, front view.
PDB entry 1np8 coloured by chain, side view.
PDB entry 1np8 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
A second binding site revealed by C-terminal truncation of calpain small subunit, a penta-EF-hand protein.
Leinala EK, Arthur JS, Grochulski P, Davies PL, Elce JS, Jia Z
Proteins 53 649-55 (2003)
PMID: 14579356

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179146 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calpain small subunit 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 18.35 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q64537 (Residues: 91-249; Coverage: 59%)
Gene names: Capn4, Capns1, Css1
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CD 13 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 58.04Å b: 67.57Å c: 75.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.196 0.239
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Crystal structure of the endophilin-A1 BAR domain.
Weissenhorn W. (2005)
3 more

1 mention without citation

Predicting the disruption by UO2(2+) of a protein-ligand interaction.
Pible et al. (2010)