› 1npp

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF AQUIFEX AEOLICUS NUSG IN P2(1)

Released:
DOI: 10.2210/pdb1npp/pdb
PDB entry 1npp coloured by chain, front view.
PDB entry 1npp coloured by chain, side view.
PDB entry 1npp coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
A spring-loaded state of NusG in its functional cycle is suggested by X-ray crystallography and supported by site-directed mutants.
Knowlton JR, Bubunenko M, Andrykovitch M, Guo W, Routzahn KM, Waugh DS, Court DL, Ji X
Biochemistry 42 2275-81 (2003)
PMID: 12600194

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription termination/antitermination protein NusG Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 248 amino acids
Theoretical weight: 28.04 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67757 (Residues: 1-248; Coverage: 100%)
Gene names: aq_1931, nusG
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21
Unit cell:
a: 87.07Å b: 55.86Å c: 112.61Å
α: 90° β: 90.39° γ: 90°
R-values:
R R work R free
0.28 0.219 0.279
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

NusG-Spt5 proteins-Universal tools for transcription modification and communication.
Tomar et al. (2013)
29 other articles

3 mentions without citation

Architecture of the RNA polymerase-Spt4/5 complex and basis of universal transcription processivity.
Martinez-Rucobo et al. (2011)
2 more