› 1npt

X-ray diffraction
2.18Å resolution

Glyceraldehyde-3-Phosphate Dehydrogenase Mutant With Cys 149 replaced by Ala complexed with NAD+

Released:
DOI: 10.2210/pdb1npt/pdb
PDB entry 1npt coloured by chain, front view.
PDB entry 1npt coloured by chain, side view.
PDB entry 1npt coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structure of two ternary complexes of phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus with NAD and D-glyceraldehyde 3-phosphate.
Didierjean C, Corbier C, Fatih M, Favier F, Boschi-Muller S, Branlant G, Aubry A
J Biol Chem 278 12968-76 (2003)
PMID: 12569100

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase Chains: O, P, Q, R
Molecule details ›
Chains: O, P, Q, R
Length: 334 amino acids
Theoretical weight: 35.96 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00362 (Residues: 2-335; Coverage: 100%)
Gene name: gap
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P3121
Unit cell:
a: 115.7Å b: 115.7Å c: 224.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.177 0.21
Expression system: Escherichia coli

Quick links

Citations

21 citation in other articles

The thioredoxin-independent isoform of chloroplastic glyceraldehyde-3-phosphate dehydrogenase is selectively regulated by glutathionylation.
Zaffagnini et al. (2007)
20 more

1 mention without citation

Hydrogen bonds of DsrD protein revealed by neutron crystallography.
Chatake et al. (2008)