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X-ray diffraction
1.75Å resolution

Structure of shikimate 5-dehydrogenase-like protein HI0607

Released:
DOI: 10.2210/pdb1npy/pdb
PDB entry 1npy coloured by chain, front view.
PDB entry 1npy coloured by chain, side view.
PDB entry 1npy coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Crystal structure of a novel shikimate dehydrogenase from Haemophilus influenzae.
Singh S, Korolev S, Koroleva O, Zarembinski T, Collart F, Joachimiak A, Christendat D
J Biol Chem 280 17101-8 (2005)
PMID: 15735308

Function and Biology Details

Reaction catalysed: 
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shikimate dehydrogenase-like protein HI_0607 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 271 amino acids
Theoretical weight: 29.97 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P44774 (Residues: 1-271; Coverage: 100%)
Gene names: HI_0607, sdhL
Sequence domains: Shikimate dehydrogenase substrate binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ACE 3 x ACE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 90.578Å b: 66.575Å c: 96.916Å
α: 90° β: 109.56° γ: 90°
R-values:
R R work R free
0.181 0.179 0.219
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The shikimate dehydrogenase family: functional diversity within a conserved structural and mechanistic framework.
Peek et al. (2015)
19 other articles

3 mentions without citation

Predicting small ligand binding sites in proteins using backbone structure.
Bordner AJ. (2008)
2 more