› 1nqb

X-ray diffraction
2Å resolution

TRIVALENT ANTIBODY FRAGMENT

Released:
DOI: 10.2210/pdb1nqb/pdb
PDB entry 1nqb coloured by chain, front view.
PDB entry 1nqb coloured by chain, side view.
PDB entry 1nqb coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The 2.0-A resolution crystal structure of a trimeric antibody fragment with noncognate VH-VL domain pairs shows a rearrangement of VH CDR3.
Pei XY, Holliger P, Murzin AG, Williams RL
Proc Natl Acad Sci U S A 94 9637-42 (1997)
PMID: 9275175

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SINGLE-CHAIN ANTIBODY FRAGMENT Chains: A, C
Molecule details ›
Chains: A, C
Length: 256 amino acids
Theoretical weight: 28.38 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: R3
Unit cell:
a: 136.32Å b: 136.32Å c: 74.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.187 0.23
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Engineered antibody fragments and the rise of single domains.
Holliger et al. (2005)
11 more

4 mentions without citation

The 2.0-A resolution crystal structure of a trimeric antibody fragment with noncognate VH-VL domain pairs shows a rearrangement of VH CDR3.
Pei et al. (1997)
3 more