› 1nqf

X-ray diffraction
2.7Å resolution

OUTER MEMBRANE COBALAMIN TRANSPORTER (BTUB) FROM E. COLI, METHIONINE SUBSTIUTION CONSTRUCT FOR SE-MET SAD PHASING

Released:
DOI: 10.2210/pdb1nqf/pdb
PDB entry 1nqf coloured by chain, front view.
PDB entry 1nqf coloured by chain, side view.
PDB entry 1nqf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Substrate-induced transmembrane signaling in the cobalamin transporter BtuB.
Chimento DP, Mohanty AK, Kadner RJ, Wiener MC
Nat Struct Biol 10 394-401 (2003)
PMID: 12652322

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138877 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vitamin B12 transporter BtuB Chain: A
Molecule details ›
Chain: A
Length: 594 amino acids
Theoretical weight: 66.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06129 (Residues: 21-614; Coverage: 100%)
Gene names: JW3938, b3966, bfe, btuB, cer, dcrC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand C8E 6 x C8E
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3121
Unit cell:
a: 81.614Å b: 81.614Å c: 226.653Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.246 0.244 0.288
Expression system: Escherichia coli

Quick links

Citations

31 review citations

Molecular basis of bacterial outer membrane permeability revisited.
Nikaido H. (2003)
30 more

5 mentions without citation

TonB-dependent transporters: regulation, structure, and function.
Noinaj et al. (2010)
4 more