› 1nqn

X-ray diffraction
1.8Å resolution

Structure of Avm-W110K (W110K mutant of avidin)

Released:
DOI: 10.2210/pdb1nqn/pdb
PDB entry 1nqn coloured by chain, front view.
PDB entry 1nqn coloured by chain, side view.
PDB entry 1nqn coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Dimer-tetramer transition between solution and crystalline states of streptavidin and avidin mutants.
Pazy Y, Eisenberg-Domovich Y, Laitinen OH, Kulomaa MS, Bayer EA, Wilchek M, Livnah O
J Bacteriol 185 4050-6 (2003)
PMID: 12837778

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Avidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 13.58 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02701 (Residues: 26-147; Coverage: 95%)
Gene name: AVD
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 67.703Å b: 77.45Å c: 42.894Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.205 0.227
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Essentials of biorecognition: the (strept)avidin-biotin system as a model for protein-protein and protein-ligand interaction.
Wilchek et al. (2006)
1 more