› 1nqo

X-ray diffraction
2.01Å resolution

Glyceraldehyde-3-Phosphate Dehydrogenase Mutant With Cys 149 Replaced By Ser Complexed With Nad+ and D-Glyceraldehyde-3-Phosphate

Released:
DOI: 10.2210/pdb1nqo/pdb
Model geometry
Fit model/data
PDB entry 1nqo coloured by chain, front view.
PDB entry 1nqo coloured by chain, side view.
PDB entry 1nqo coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structure of two ternary complexes of phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus with NAD and D-glyceraldehyde 3-phosphate.
Didierjean C, Corbier C, Fatih M, Favier F, Boschi-Muller S, Branlant G, Aubry A
J Biol Chem 278 12968-76 (2003)
PMID: 12569100

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, C, O, Q
Molecule details ›
Chains: A, C, O, Q
Length: 334 amino acids
Theoretical weight: 35.97 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00362 (Residues: 2-335; Coverage: 100%)
Gene name: gap
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
Ligand G3H 4 x G3H
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ENRAF-NONIUS FR591
Spacegroup: C2
Unit cell:
a: 140.306Å b: 87.838Å c: 119.41Å
α: 90° β: 119.05° γ: 90°
R-values:
R R work R free
0.194 0.193 0.238
Expression system: Escherichia coli

Quick links

Citations

19 citation in other articles

The thioredoxin-independent isoform of chloroplastic glyceraldehyde-3-phosphate dehydrogenase is selectively regulated by glutathionylation.
Zaffagnini et al. (2007)
18 more

6 mentions without citation

Oxidatively modified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Alzheimer's disease: many pathways to neurodegeneration.
Butterfield et al. (2010)
5 more