› 1nrk

X-ray diffraction
2.8Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142353 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA-modifying protein YgfZ Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 36.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADE8 (Residues: 1-325; Coverage: 100%)
Gene names: JW2866, b2898, ygfZ, yzzW
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: P3121
Unit cell:
a: 151Å b: 151Å c: 68Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.193 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Toward a better understanding of folate metabolism in health and disease.
Zheng et al. (2019)
3 more

3 mentions without citation

Crystal structure of the YgfZ protein from Escherichia coli suggests a folate-dependent regulatory role in one-carbon metabolism.
Teplyakov et al. (2004)
2 more