› 1nt0

X-ray diffraction
2.7Å resolution

Crystal structure of the CUB1-EGF-CUB2 region of MASP2

Released:
DOI: 10.2210/pdb1nt0/pdb
PDB entry 1nt0 coloured by chain, front view.
PDB entry 1nt0 coloured by chain, side view.
PDB entry 1nt0 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of the CUB1-EGF-CUB2 region of mannose-binding protein associated serine protease-2.
Feinberg H, Uitdehaag JC, Davies JM, Wallis R, Drickamer K, Weis WI
EMBO J 22 2348-59 (2003)
PMID: 12743029

Function and Biology Details

Reaction catalysed: 
Selective cleavage after Arg(223) in complement component C2 (-Ser-Leu-Gly-Arg-|-Lys-Ile-Gln-Ile) and after Arg(76) in complement component C4 (-Gly-Leu-Gln-Arg-|-Ala-Leu-Glu-Ile)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannan-binding lectin serine protease 2 A chain Chains: A, G
Molecule details ›
Chains: A, G
Length: 286 amino acids
Theoretical weight: 32.56 KDa
Source organism: Rattus norvegicus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q9JJS8 (Residues: 20-299; Coverage: 42%)
Gene name: Masp2
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NAG 2 x NAG
Ligand CA 2 x CA
Ligand EDO 2 x EDO
1 modified residue:
Ligand AHB 2 x AHB

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 70.411Å b: 103.901Å c: 70.484Å
α: 90° β: 119.93° γ: 90°
R-values:
R R work R free
0.248 0.248 0.283
Expression system: Cricetulus griseus

Quick links

Citations

23 review citations

The lectin-complement pathway--its role in innate immunity and evolution.
Fujita et al. (2004)
22 more

8 mentions without citation

Structural studies of neuropilin/antibody complexes provide insights into semaphorin and VEGF binding.
Appleton et al. (2007)
7 more