› 1ntb

X-ray diffraction
2.9Å resolution

2.9 A crystal structure of Streptomycin RNA-aptamer complex

Released:
DOI: 10.2210/pdb1ntb/pdb
PDB entry 1ntb coloured by chain, front view.
PDB entry 1ntb coloured by chain, side view.
PDB entry 1ntb coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Encapsulating streptomycin within a small 40-mer RNA.
Tereshko V, Skripkin E, Patel DJ
Chem Biol 10 175-87 (2003)
PMID: 12618190

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196144 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
5'-R(*GP*GP*AP*UP*CP*GP*CP*AP*UP*UP*UP*GP*GP*AP*CP*UP*UP*CP*UP*GP*CP*C)-3' Chain: A
Molecule details ›
Chain: A
Length: 22 nucleotides
Theoretical weight: 6.99 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-R(*CP*GP*GP*CP*AP*CP*CP*AP*CP*GP*GP*UP*CP*GP*GP*AP*UP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 18 nucleotides
Theoretical weight: 5.76 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand SRY 1 x SRY
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: I4
Unit cell:
a: 82.634Å b: 82.634Å c: 49.153Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.255
Expression system: Not provided

Quick links

Citations

12 review citations

Analytical applications of aptamers.
Tombelli et al. (2005)
11 more

13 mentions without citation

The building blocks and motifs of RNA architecture.
Leontis et al. (2006)
12 more