› 1nu0

X-ray diffraction
1.6Å resolution

Structure of the double mutant (L6M; F134M, SeMet form) of yqgF from Escherichia coli, a hypothetical protein

Released:
DOI: 10.2210/pdb1nu0/pdb
PDB entry 1nu0 coloured by chain, front view.
PDB entry 1nu0 coloured by chain, side view.
PDB entry 1nu0 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Galkin A, Sarikaya E, Krajewski W, Howard A, Herzberg O, Structure 2 Function Project (S2F)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative pre-16S rRNA nuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 138 amino acids
Theoretical weight: 15.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8I1 (Residues: 1-138; Coverage: 100%)
Gene names: JW2916, b2949, ruvX, yqgF
Sequence domains: Holliday junction resolvase
Structure domains: YqgF/RNase H-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 58.5Å b: 59Å c: 67.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.228
Expression system: Escherichia coli

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