› 1nvg

X-ray diffraction
2.5Å resolution

N249Y MUTANT OF THE ALCOHOL DEHYDROGENASE FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS-TETRAGONAL CRYSTAL FORM

Released:
DOI: 10.2210/pdb1nvg/pdb
PDB entry 1nvg coloured by chain, front view.
PDB entry 1nvg coloured by chain, side view.
PDB entry 1nvg coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Structural study of a single-point mutant of Sulfolobus solfataricus alcohol dehydrogenase with enhanced activity.
Esposito L, Bruno I, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A
FEBS Lett 539 14-8 (2003)
PMID: 12650918

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent alcohol dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 347 amino acids
Theoretical weight: 37.66 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P39462 (Residues: 1-347; Coverage: 100%)
Gene names: SSO2536, adh
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: I4122
Unit cell:
a: 125.396Å b: 125.396Å c: 117.186Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.196 0.236
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Characterization of a zinc-containing alcohol dehydrogenase with stereoselectivity from the hyperthermophilic archaeon Thermococcus guaymasensis.
Ying et al. (2011)
5 more

1 mention without citation

Atomic structures suggest determinants of transmission barriers in mammalian prion disease.
Apostol et al. (2011)