› 1nxu

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF E. COLI HYPOTHETICAL OXIDOREDUCTASE YIAK NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER82.

Released:
DOI: 10.2210/pdb1nxu/pdb
PDB entry 1nxu coloured by chain, front view.
PDB entry 1nxu coloured by chain, side view.
PDB entry 1nxu coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A novel NAD-binding protein revealed by the crystal structure of 2,3-diketo-L-gulonate reductase (YiaK).
Forouhar F, Lee I, Benach J, Kulkarni K, Xiao R, Acton TB, Montelione GT, Tong L
J Biol Chem 279 13148-55 (2004)
PMID: 14718529

Function and Biology Details

Reaction catalysed: 
3-dehydro-L-gulonate + NAD(P)(+) = (4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153464 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,3-diketo-L-gulonate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 333 amino acids
Theoretical weight: 37.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P37672 (Residues: 1-332; Coverage: 100%)
Gene names: JW3547, b3575, dlgD, yiaK
Sequence domains: Malate/L-lactate dehydrogenase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 58.012Å b: 51.229Å c: 108.93Å
α: 90° β: 103.78° γ: 90°
R-values:
R R work R free
0.2 0.192 0.235
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Vitamin C. Biosynthesis, recycling and degradation in mammals.
Linster et al. (2007)
1 more

1 mention without citation

Using neural networks and evolutionary information in decoy discrimination for protein tertiary structure prediction.
Tan et al. (2008)