› 1ny5

X-ray diffraction
2.4Å resolution

Crystal structure of sigm54 activator (AAA+ ATPase) in the inactive state

Released:
DOI: 10.2210/pdb1ny5/pdb
PDB entry 1ny5 coloured by chain, front view.
PDB entry 1ny5 coloured by chain, side view.
PDB entry 1ny5 coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Regulation of the transcriptional activator NtrC1: structural studies of the regulatory and AAA+ ATPase domains.
Lee SY, De La Torre A, Yan D, Kustu S, Nixon BT, Wemmer DE
Genes Dev 17 2552-63 (2003)
PMID: 14561776

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator (NtrC family) Chains: A, B
Molecule details ›
Chains: A, B
Length: 387 amino acids
Theoretical weight: 44.3 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67198 (Residues: 1-387; Coverage: 88%)
Gene names: aq_1117, ntrC1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand PO4 4 x PO4
Ligand ADP 2 x ADP
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3221
Unit cell:
a: 94.765Å b: 94.765Å c: 195.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.213 0.257
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Evolutionary relationships and structural mechanisms of AAA+ proteins.
Erzberger et al. (2006)
25 more

28 mentions without citation

Diversity of structure and function of response regulator output domains.
Galperin MY. (2010)
27 more