› 1nyh

X-ray diffraction
3.1Å resolution

Crystal Structure of the Coiled-coil Dimerization Motif of Sir4

Released:
DOI: 10.2210/pdb1nyh/pdb
PDB entry 1nyh coloured by chain, front view.
PDB entry 1nyh coloured by chain, side view.
PDB entry 1nyh coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the coiled-coil dimerization motif of Sir4 and its interaction with Sir3.
Chang JF, Hall BE, Tanny JC, Moazed D, Filman D, Ellenberger T
Structure 11 637-49 (2003)
PMID: 12791253

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein SIR4 Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11978 (Residues: 1198-1358; Coverage: 12%)
Gene names: ASD1, SIR4, STE9, UTH2, YD9934.12, YDR227W
Structure domains: Single helix bin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P6522
Unit cell:
a: 108.64Å b: 108.64Å c: 74.75Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.273 0.271 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

SIR proteins and the assembly of silent chromatin in budding yeast.
Kueng et al. (2013)
8 more

3 mentions without citation

The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.
Gartenberg et al. (2016)
2 more