› 1nz0

X-ray diffraction
1.2Å resolution

RNASE P PROTEIN FROM THERMOTOGA MARITIMA

Released:
DOI: 10.2210/pdb1nz0/pdb
PDB entry 1nz0 coloured by chain, front view.
PDB entry 1nz0 coloured by chain, side view.
PDB entry 1nz0 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
High-resolution structure of RNase P protein from Thermotoga maritima.
Kazantsev AV, Krivenko AA, Harrington DJ, Carter RJ, Holbrook SR, Adams PD, Pace NR
Proc Natl Acad Sci U S A 100 7497-502 (2003)
PMID: 12799461

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
homo trimer
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-194798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease P protein component Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 118 amino acids
Theoretical weight: 14.43 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X1H4 (Residues: 1-117; Coverage: 100%)
Gene names: TM_1463, rnpA
Sequence domains: Ribonuclease P
Structure domains: Ribosomal Protein S5; domain 2

Ligands and Environments

1 bound ligand:
Ligand SO4 16 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 56.227Å b: 64.143Å c: 68.326Å
α: 90° β: 102.02° γ: 90°
R-values:
R R work R free
0.163 0.162 0.217
Expression system: Escherichia coli

Quick links

Citations

9 review citations

RNase P: interface of the RNA and protein worlds.
Evans et al. (2006)
8 more

15 mentions without citation

Of proteins and RNA: the RNase P/MRP family.
Esakova et al. (2010)
14 more