› 1nz9

Solution NMR

Solution Structure of the N-utilization substance G (NusG) C-terminal (NGC) domain from Thermus thermophilus

Released:
DOI: 10.2210/pdb1nz9/pdb
PDB entry 1nz9 coloured by chain, ensemble of 31 models, front view.
PDB entry 1nz9 coloured by chain, ensemble of 31 models, side view.
PDB entry 1nz9 coloured by chain, ensemble of 31 models, top view.
Source organism: Thermus thermophilus
Primary publication:
Structural and sequence comparisons arising from the solution structure of the transcription elongation factor NusG from Thermus thermophilus.
Reay P, Yamasaki K, Terada T, Kuramitsu S, Shirouzu M, Yokoyama S
Proteins 56 40-51 (2004)
PMID: 15162485

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription termination/antitermination protein NusG Chain: A
Molecule details ›
Chain: A
Length: 58 amino acids
Theoretical weight: 6.36 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35872 (Residues: 127-184; Coverage: 32%)
Gene names: TTHA0248, nusG
Sequence domains: KOW motif
Structure domains: SH3 type barrels.

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, molecular dynamics, torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

NusG-Spt5 proteins-Universal tools for transcription modification and communication.
Tomar et al. (2013)
1 more

2 mentions without citation

Two structurally independent domains of E. coli NusG create regulatory plasticity via distinct interactions with RNA polymerase and regulators.
Mooney et al. (2009)
1 more