› 1nzf

X-ray diffraction
2.1Å resolution

T4 phage BGT-D100A mutant in complex with UDP-glucose: Form II

Released:
DOI: 10.2210/pdb1nzf/pdb
Model geometry
Fit model/data
PDB entry 1nzf coloured by chain, front view.
PDB entry 1nzf coloured by chain, side view.
PDB entry 1nzf coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Crystal structures of the T4 phage beta-glucosyltransferase and the D100A mutant in complex with UDP-glucose: glucose binding and identification of the catalytic base for a direct displacement mechanism.
Larivière L, Gueguen-Chaignon V, Moréra S
J Mol Biol 330 1077-86 (2003)
PMID: 12860129

Function and Biology Details

Reaction catalysed: 
Transfers a beta-D-glucosyl residue from UDP-alpha-D-glucose to a hydroxymethylcytosine residue in DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA beta-glucosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 40.68 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04547 (Residues: 1-351; Coverage: 100%)
Gene name: bgt
Sequence domains: Bacteriophage T4 beta-glucosyltransferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand UPG 1 x UPG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 63.119Å b: 72.958Å c: 80.148Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.22
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
7 more