Function and Biology Details
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
SF1-U2AF65 splicing factor complex (preferred)
PDBe Complex ID:
PDB-CPX-150720 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Length: 104 amino acids
Theoretical weight: 11.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P26368 (Residues: 372-475; Coverage: 22%)
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Splicing factor 1
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.69 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
Length: 13 amino acids
Theoretical weight: 1.69 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
- Canonical: Q15637 (Residues: 13-25; Coverage: 2%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Refinement method:
Restrained molecular dynamics using CNS and ARIA for ambiguous distance restraints
Expression systems:
- Escherichia coli BL21(DE3)
- Not provided
