› 1o2f

Solution NMR

COMPLEX OF ENZYME IIAGLC AND IIBGLC PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI NMR, RESTRAINED REGULARIZED MEAN STRUCTURE

Released:
DOI: 10.2210/pdb1o2f/pdb
PDB entry 1o2f coloured by chain, ensemble of 3 models, front view.
PDB entry 1o2f coloured by chain, ensemble of 3 models, side view.
PDB entry 1o2f coloured by chain, ensemble of 3 models, top view.
Source organism: Escherichia coli
Primary publication:
Solution structure of the phosphoryl transfer complex between the signal-transducing protein IIAGlucose and the cytoplasmic domain of the glucose transporter IICBGlucose of the Escherichia coli glucose phosphotransferase system.
Cai M, Williams DC, Wang G, Lee BR, Peterkofsky A, Clore GM
J Biol Chem 278 25191-206 (2003)
PMID: 12716891

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159658 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PTS system glucose-specific EIIA component Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 18.14 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69783 (Residues: 2-169; Coverage: 99%)
Gene names: JW2410, b2417, crr, gsr, iex, tgs, treD
Sequence domains: phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1
Structure domains: Glucose Permease (Domain IIA)
PTS system glucose-specific EIICB component Chain: B
Molecule details ›
Chain: B
Length: 90 amino acids
Theoretical weight: 9.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69786 (Residues: 387-476; Coverage: 19%)
Gene names: JW1087, b1101, glcA, ptsG, umg
Sequence domains: phosphotransferase system, EIIB
Structure domains: Glucose permease domain IIB

Ligands and Environments

1 bound ligand:
Ligand PO3 1 x PO3
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: CONJOINED RIGID BODY/TORSION ANGLE DYNAMICS
Expression system: Escherichia coli

Quick links

Citations

11 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
10 more

11 mentions without citation

Protein-protein docking benchmark version 4.0.
Hwang et al. (2010)
10 more