› 1o3s

X-ray diffraction
3Å resolution

PROTEIN-DNA RECOGNITION AND DNA DEFORMATION REVEALED IN CRYSTAL STRUCTURES OF CAP-DNA COMPLEXES

Released:
DOI: 10.2210/pdb1o3s/pdb
PDB entry 1o3s coloured by chain, front view.
PDB entry 1o3s coloured by chain, side view.
PDB entry 1o3s coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Indirect readout of DNA sequence at the primary-kink site in the CAP-DNA complex: alteration of DNA binding specificity through alteration of DNA kinking.
Chen S, Gunasekera A, Zhang X, Kunkel TA, Ebright RH, Berman HM
J Mol Biol 314 75-82 (2001)
PMID: 11724533

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114294 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding transcriptional dual regulator CRP Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 9-208; Coverage: 95%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:
5'-D(*AP*AP*AP*AP*AP*TP*GP*CP*GP*AP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.39 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*CP*TP*AP*GP*AP*TP*CP*GP*CP*AP*TP*TP*TP*TP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.55 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CMP 2 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 78.05Å b: 78.05Å c: 142.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.26 0.26 0.308
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Protein-DNA recognition patterns and predictions.
Sarai et al. (2005)
36 other articles

7 mentions without citation

Catabolite activator protein: DNA binding and transcription activation.
Lawson et al. (2004)
6 more