› 1o3t

X-ray diffraction
2.8Å resolution

PROTEIN-DNA RECOGNITION AND DNA DEFORMATION REVEALED IN CRYSTAL STRUCTURES OF CAP-DNA COMPLEXES

Released:
DOI: 10.2210/pdb1o3t/pdb
PDB entry 1o3t coloured by chain, front view.
PDB entry 1o3t coloured by chain, side view.
PDB entry 1o3t coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Indirect readout of DNA sequence at the primary-kink site in the CAP-DNA complex: DNA binding specificity based on energetics of DNA kinking.
Chen S, Vojtechovsky J, Parkinson GN, Ebright RH, Berman HM
J Mol Biol 314 63-74 (2001)
PMID: 11724532

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114295 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding transcriptional dual regulator CRP Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 22.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 9-208; Coverage: 95%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:
5'-D(*GP*CP*GP*AP*AP*AP*AP*AP*TP*GP*CP*GP*AP*T)-3' Chains: C, F
Molecule details ›
Chains: C, F
Length: 14 nucleotides
Theoretical weight: 4.34 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*CP*TP*AP*GP*AP*TP*CP*GP*CP*AP*TP*TP*TP*TP*TP*CP*G)-3' Chains: D, E
Molecule details ›
Chains: D, E
Length: 17 nucleotides
Theoretical weight: 5.17 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CMP 2 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2221
Unit cell:
a: 136.99Å b: 152.8Å c: 76.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.303
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Catabolite activator protein: DNA binding and transcription activation.
Lawson et al. (2004)
1 more

10 mentions without citation

Post-translational Lysine Ac(et)ylation in Bacteria: A Biochemical, Structural, and Synthetic Biological Perspective.
Lammers M. (2021)
9 more