› 1o54

X-ray diffraction
1.65Å resolution

Crystal structure of SAM-dependent O-methyltransferase (TM0748) from Thermotoga maritima at 1.65 A resolution

Released:
DOI: 10.2210/pdb1o54/pdb
PDB entry 1o54 coloured by chain, front view.
PDB entry 1o54 coloured by chain, side view.
PDB entry 1o54 coloured by chain, top view.
Source organism: Thermotoga maritima
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(58) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194617 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (adenine(58)-N(1))-methyltransferase TrmI Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.58 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZK7 (Residues: 2-265; Coverage: 100%)
Gene name: TM_0748
Sequence domains: tRNA methyltransferase complex GCD14 subunit
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I222
Unit cell:
a: 64.253Å b: 74.992Å c: 143.701Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.158 0.188
Expression system: Escherichia coli

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