› 1o5z

X-ray diffraction
2.1Å resolution

Crystal structure of Folylpolyglutamate synthase (TM0166) from Thermotoga maritima at 2.10 A resolution

Released:
DOI: 10.2210/pdb1o5z/pdb
PDB entry 1o5z coloured by chain, front view.
PDB entry 1o5z coloured by chain, side view.
PDB entry 1o5z coloured by chain, top view.
Source organism: Thermotoga maritima
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194452 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tetrahydrofolate synthase Chain: A
Molecule details ›
Chain: A
Length: 442 amino acids
Theoretical weight: 50.4 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WY13 (Residues: 1-430; Coverage: 100%)
Gene name: TM_0166
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand CL 2 x CL
Ligand UNL 2 x UNL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P6122
Unit cell:
a: 84.751Å b: 84.751Å c: 280.679Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.181 0.244
Expression system: Escherichia coli

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