› 1o6s

X-ray diffraction
1.8Å resolution

Internalin (Listeria monocytogenes) / E-Cadherin (human) Recognition Complex

Released:
DOI: 10.2210/pdb1o6s/pdb
PDB entry 1o6s coloured by chain, front view.
PDB entry 1o6s coloured by chain, side view.
PDB entry 1o6s coloured by chain, top view.
Source organisms:
Primary publication:
Structure of internalin, a major invasion protein of Listeria monocytogenes, in complex with its human receptor E-cadherin.
Schubert WD, Urbanke C, Ziehm T, Beier V, Machner MP, Domann E, Wehland J, Chakraborty T, Heinz DW
Cell 111 825-36 (2002)
PMID: 12526809

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143596 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Internalin A Chain: A
Molecule details ›
Chain: A
Length: 466 amino acids
Theoretical weight: 50.24 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DJM0 (Residues: 35-496; Coverage: 60%)
Gene names: inlA, lmo0433
Sequence domains:
Structure domains:
Cadherin-1 Chain: B
Molecule details ›
Chain: B
Length: 105 amino acids
Theoretical weight: 11.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P12830 (Residues: 155-255; Coverage: 12%)
Gene names: CDH1, CDHE, UVO
Sequence domains: Cadherin domain
Structure domains: Cadherins

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 55.378Å b: 86.859Å c: 110.723Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.168 0.221
Expression system: Escherichia coli BL21

Quick links

Citations

56 review citations

Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria.
Marraffini et al. (2006)
55 more

22 mentions without citation

Neurexins, neuroligins and LRRTMs: synaptic adhesion getting fishy.
Wright et al. (2011)
21 more