› 1o9b

X-ray diffraction
2.5Å resolution

QUINATE/SHIKIMATE DEHYDROGENASE YDIB COMPLEXED WITH NADH

Released:
DOI: 10.2210/pdb1o9b/pdb
PDB entry 1o9b coloured by chain, front view.
PDB entry 1o9b coloured by chain, side view.
PDB entry 1o9b coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structures of shikimate dehydrogenase AroE and its Paralog YdiB. A common structural framework for different activities.
Michel G, Roszak AW, Sauvé V, Maclean J, Matte A, Coggins JR, Cygler M, Lapthorn AJ
J Biol Chem 278 19463-72 (2003)
PMID: 12637497

Function and Biology Details

Reaction catalysed: 
L-quinate + NAD(P)(+) = 3-dehydroquinate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141299 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Quinate/shikimate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 288 amino acids
Theoretical weight: 31.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6D5 (Residues: 1-288; Coverage: 100%)
Gene name: ydiB
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 2 x NAI
1 bound ligand:
Ligand PO4 5 x PO4
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P64
Unit cell:
a: 157.874Å b: 157.874Å c: 40.011Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.226 0.294
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The shikimate pathway: review of amino acid sequence, function and three-dimensional structures of the enzymes.
Mir et al. (2015)
3 more

7 mentions without citation

Contribution of structural genomics to understanding the biology of Escherichia coli.
Matte et al. (2003)
6 more