› 1oaq

X-ray diffraction
1.5Å resolution

Free conformation Ab1 of the IgE SPE-7

Released:
DOI: 10.2210/pdb1oaq/pdb
PDB entry 1oaq coloured by chain, front view.
PDB entry 1oaq coloured by chain, side view.
PDB entry 1oaq coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Antibody multispecificity mediated by conformational diversity.
James LC, Roversi P, Tawfik DS
Science 299 1362-7 (2003)
PMID: 12610298

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208473 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 121 amino acids
Theoretical weight: 13.62 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Ig lambda-1 chain V regions MOPC 104E/RPC20/J558/S104 Chain: L
Molecule details ›
Chain: L
Length: 120 amino acids
Theoretical weight: 12.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01724 (Residues: 20-129; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 36.541Å b: 70.743Å c: 82.121Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.16 0.182
Expression system: Escherichia coli

Quick links

Citations

95 review citations

Engineered antibody fragments and the rise of single domains.
Holliger et al. (2005)
94 more

11 mentions without citation

FiberDock: Flexible induced-fit backbone refinement in molecular docking.
Mashiach et al. (2010)
10 more