› 1oax

X-ray diffraction
2.67Å resolution

Fv Structure of the IgE SPE-7 in complex with acenaphthenequinone

Released:
DOI: 10.2210/pdb1oax/pdb
PDB entry 1oax coloured by chain, front view.
PDB entry 1oax coloured by chain, side view.
PDB entry 1oax coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Antibody multispecificity mediated by conformational diversity.
James LC, Roversi P, Tawfik DS
Science 299 1362-7 (2003)
PMID: 12610298

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-208476 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
IMMUNOGLOBULIN E Chains: H, J
Molecule details ›
Chains: H, J
Length: 122 amino acids
Theoretical weight: 13.67 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Ig lambda-1 chain V regions MOPC 104E/RPC20/J558/S104 Chains: L, M, N, O
Molecule details ›
Chains: L, M, N, O
Length: 110 amino acids
Theoretical weight: 11.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01724 (Residues: 20-128; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ANQ 2 x ANQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 78.726Å b: 78.636Å c: 167.564Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.282
Expression system: Escherichia coli

Quick links

Citations

95 review citations

Engineered antibody fragments and the rise of single domains.
Holliger et al. (2005)
94 more

4 mentions without citation

The specificity of cross-reactivity: promiscuous antibody binding involves specific hydrogen bonds rather than nonspecific hydrophobic stickiness.
James et al. (2003)
3 more