› 1ocs

X-ray diffraction
2.03Å resolution

Crystal structure of the yeast PX-doamin protein Grd19p (sorting nexin3) complexed to phosphatidylinosytol-3-phosphate.

Released:
DOI: 10.2210/pdb1ocs/pdb
PDB entry 1ocs coloured by chain, front view.
PDB entry 1ocs coloured by chain, side view.
PDB entry 1ocs coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of the yeast Phox homology (PX) domain protein Grd19p complexed to phosphatidylinositol-3-phosphate.
Zhou CZ, Li de La Sierra-Gallay I, Quevillon-Cheruel S, Collinet B, Minard P, Blondeau K, Henckes G, Aufrère R, Leulliot N, Graille M, Sorel I, Savarin P, de la Torre F, Poupon A, Janin J, van Tilbeurgh H
J Biol Chem 278 50371-6 (2003)
PMID: 14514667

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sorting nexin-3 Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.88 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q08826 (Residues: 1-162; Coverage: 100%)
Gene names: GRD19, SNX3, YOR357C
Sequence domains: PX domain
Structure domains: Phox-like domain

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P6122
Unit cell:
a: 55.73Å b: 55.73Å c: 187.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Membrane-protein interactions in cell signaling and membrane trafficking.
Cho et al. (2005)
6 more

6 mentions without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)
5 more