1odi

X-ray diffraction
2.4Å resolution

Purine nucleoside phosphorylase from Thermus Thermophilus

Released:

Function and Biology Details

Reaction catalysed:
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177954 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 235 amino acids
Theoretical weight: 25.44 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SID9 (Residues: 1-235; Coverage: 100%)
Gene name: TTHA1435
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-D
Spacegroup: P43212
Unit cell:
a: 132.365Å b: 132.365Å c: 171.009Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.234
Expression system: Escherichia coli BL21(DE3)