› 1ogk

X-ray diffraction
2.85Å resolution

The crystal structure of Trypanosoma cruzi dUTPase in complex with dUDP

Released:
DOI: 10.2210/pdb1ogk/pdb
PDB entry 1ogk coloured by chain, front view.
PDB entry 1ogk coloured by chain, side view.
PDB entry 1ogk coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
The crystal structure of Trypanosoma cruzi dUTPase reveals a novel dUTP/dUDP binding fold.
Harkiolaki M, Dodson EJ, Bernier-Villamor V, Turkenburg JP, González-Pacanowska D, Wilson KS
Structure 12 41-53 (2004)
PMID: 14725764

Function and Biology Details

Reaction catalysed: 
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative deoxyuridine triphosphatase Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 283 amino acids
Theoretical weight: 32.1 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15923 (Residues: 1-283; Coverage: 100%)
Gene names: C3747_54g158, Tcdut
Sequence domains: dUTPase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DUD 3 x DUD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 106.386Å b: 50.307Å c: 111.387Å
α: 90° β: 101.35° γ: 90°
R-values:
R R work R free
0.209 0.205 0.273
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

DNA base damage recognition and removal: new twists and grooves.
Huffman et al. (2005)
7 more

5 mentions without citation

Copaifera of the Neotropics: A Review of the Phytochemistry and Pharmacology.
da Trindade et al. (2018)
4 more