› 1oh0

X-ray diffraction
1.1Å resolution

CRYSTAL STRUCTURE OF KETOSTEROID ISOMERASE COMPLEXED WITH EQUILENIN

Released:
DOI: 10.2210/pdb1oh0/pdb
PDB entry 1oh0 coloured by chain, front view.
PDB entry 1oh0 coloured by chain, side view.
PDB entry 1oh0 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
High-resolution crystal structures of delta5-3-ketosteroid isomerase with and without a reaction intermediate analogue.
Kim SW, Cha SS, Cho HS, Kim JS, Ha NC, Cho MJ, Joo S, Kim KK, Choi KY, Oh BH
Biochemistry 36 14030-6 (1997)
PMID: 9369474

Function and Biology Details

Reaction catalysed: 
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139594 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid Delta-isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 14.55 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07445 (Residues: 1-131; Coverage: 100%)
Gene name: ksi
Sequence domains: SnoaL-like domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

2 bound ligands:
Ligand EQU 2 x EQU
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: C2
Unit cell:
a: 86.334Å b: 72.044Å c: 50.708Å
α: 90° β: 90.03° γ: 90°
R-values:
R R work R free
0.216 0.216 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

How far divergent evolution goes in proteins.
Murzin AG. (1998)
7 more

23 mentions without citation

Enzyme architecture: on the importance of being in a protein cage.
Richard et al. (2014)
22 more