› 1ohw

X-ray diffraction
2.3Å resolution

4-AMINOBUTYRATE-AMINOTRANSFERASE inactivated by gamma-vinyl GABA

Released:
DOI: 10.2210/pdb1ohw/pdb
Model geometry
Fit model/data
PDB entry 1ohw coloured by chain, front view.
PDB entry 1ohw coloured by chain, side view.
PDB entry 1ohw coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structures of gamma-aminobutyric acid (GABA) aminotransferase, a pyridoxal 5'-phosphate, and [2Fe-2S] cluster-containing enzyme, complexed with gamma-ethynyl-GABA and with the antiepilepsy drug vigabatrin.
Storici P, De Biase D, Bossa F, Bruno S, Mozzarelli A, Peneff C, Silverman RB, Schirmer T
J Biol Chem 279 363-73 (2004)
PMID: 14534310

Function and Biology Details

Reactions catalysed: 
4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.
(S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160351 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-aminobutyrate aminotransferase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 472 amino acids
Theoretical weight: 53.37 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P80147 (Residues: 29-500; Coverage: 94%)
Gene names: ABAT, GABAT
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
2 bound ligands:
Ligand VIG 4 x VIG
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 69.66Å b: 226.712Å c: 71.366Å
α: 90° β: 108.77° γ: 90°
R-values:
R R work R free
0.19 0.188 0.215

Quick links

Citations

12 review citations

Bioinformatic analysis of a PLP-dependent enzyme superfamily suitable for biocatalytic applications.
Steffen-Munsberg et al. (2015)
11 more

10 mentions without citation

Design and Mechanism of GABA Aminotransferase Inactivators. Treatments for Epilepsies and Addictions.
Silverman RB. (2018)
9 more