› 1oih

X-ray diffraction
1.89Å resolution

Crystal structure of the alkylsulfatase AtsK, a non-heme Fe(II) alphaketoglutarate dependent Dioxygenase

Released:
DOI: 10.2210/pdb1oih/pdb
PDB entry 1oih coloured by chain, front view.
PDB entry 1oih coloured by chain, side view.
PDB entry 1oih coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Crystal structure of the alkylsulfatase AtsK: insights into the catalytic mechanism of the Fe(II) alpha-ketoglutarate-dependent dioxygenase superfamily.
Müller I, Kahnert A, Pape T, Sheldrick GM, Meyer-Klaucke W, Dierks T, Kertesz M, Usón I
Biochemistry 43 3075-88 (2004)
PMID: 15023059

Function and Biology Details

Reaction catalysed: 
A primary alkyl sulfate ester + 2-oxoglutarate + O(2) = an aldehyde + succinate + CO(2) + sulfate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194416 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-ketoglutarate-dependent sulfate ester dioxygenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 301 amino acids
Theoretical weight: 33.25 KDa
Source organism: Pseudomonas putida
UniProt:
  • Canonical: Q9WWU5 (Residues: 1-301; Coverage: 100%)
Gene name: atsK
Sequence domains: Taurine catabolism dioxygenase TauD, TfdA family
Structure domains: Clavaminate synthase-like

Ligands and Environments

1 bound ligand:
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 72.242Å b: 145.344Å c: 159.457Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.191

Quick links

Citations

12 review citations

Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins.
Clifton et al. (2006)
11 more

1 mention without citation

Mycobacterium tuberculosis Rv3406 is a type II alkyl sulfatase capable of sulfate scavenging.
Sogi et al. (2013)