› 1ojl

X-ray diffraction
3Å resolution

Crystal structure of a sigma54-activator suggests the mechanism for the conformational switch necessary for sigma54 binding

Released:
DOI: 10.2210/pdb1ojl/pdb
PDB entry 1ojl coloured by chain, front view.
PDB entry 1ojl coloured by chain, side view.
PDB entry 1ojl coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Crystal structure of the central and C-terminal domain of the sigma(54)-activator ZraR.
Sallai L, Tucker PA
J Struct Biol 151 160-70 (2005)
PMID: 16005641

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150601 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulatory protein ZraR Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 304 amino acids
Theoretical weight: 33.74 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P25852 (Residues: 141-441; Coverage: 68%)
Gene names: STM4174, STMF1.27, hydG, zraR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 5 x PO4
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P2221
Unit cell:
a: 107.44Å b: 114.74Å c: 187.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.252 0.308
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
9 more

6 mentions without citation

Diversity of structure and function of response regulator output domains.
Galperin MY. (2010)
5 more