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X-ray diffraction
2.28Å resolution

crystal structure of the essential E. coli YeaZ protein by MAD method using the gadolinium complex "DOTMA"

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-160068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 251 amino acids
Theoretical weight: 27.61 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76256 (Residues: 1-231; Coverage: 100%)
Gene names: JW1796, b1807, tsaB, yeaZ
Sequence domains: tRNA N6-adenosine threonylcarbamoyltransferase
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 76.34Å b: 97.6Å c: 141.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.257
Expression system: Escherichia coli BL21(DE3)