› 1olo

X-ray diffraction
2.55Å resolution

Hexameric Replicative DNA Helicase RepA from Plasmid RSF1010 - Cubic Crystal Structure

Released:
DOI: 10.2210/pdb1olo/pdb
PDB entry 1olo coloured by chain, front view.
PDB entry 1olo coloured by chain, side view.
PDB entry 1olo coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Hexameric RSF1010 helicase RepA: the structural and functional importance of single amino acid residues.
Ziegelin G, Niedenzu T, Lurz R, Saenger W, Lanka E
Nucleic Acids Res 31 5917-29 (2003)
PMID: 14530440

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein RepA Chains: A, B
Molecule details ›
Chains: A, B
Length: 279 amino acids
Theoretical weight: 29.95 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P20356 (Residues: 1-279; Coverage: 100%)
Gene name: repA
Sequence domains: AAA domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P4332
Unit cell:
a: 190.293Å b: 190.293Å c: 190.293Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.211
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Roles of Pif1-like helicases in the maintenance of genomic stability.
Boulé et al. (2006)
3 more

1 mention without citation

Hexameric RSF1010 helicase RepA: the structural and functional importance of single amino acid residues.
Ziegelin et al. (2003)