› 1olr

X-ray diffraction
1.2Å resolution

The Humicola grisea Cel12A Enzyme Structure at 1.2 A Resolution

Released:
DOI: 10.2210/pdb1olr/pdb
PDB entry 1olr coloured by chain, front view.
PDB entry 1olr coloured by chain, side view.
PDB entry 1olr coloured by chain, top view.
Source organism: Trichocladium griseum
Primary publication:
The Humicola grisea Cel12A enzyme structure at 1.2 A resolution and the impact of its free cysteine residues on thermal stability.
Sandgren M, Gualfetti PJ, Paech C, Paech S, Shaw A, Gross LS, Saldajeno M, Berglund GI, Jones TA, Mitchinson C
Protein Sci 12 2782-93 (2003)
PMID: 14627738

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 25.89 KDa
Source organism: Trichocladium griseum
Expression system: Aspergillus niger
UniProt:
  • Canonical: Q8NJY3 (Residues: 31-254; Coverage: 95%)
Gene name: cel12A
Sequence domains: Glycosyl hydrolase family 12
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P43212
Unit cell:
a: 49.231Å b: 49.231Å c: 165.517Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.135 0.135 0.151
Expression system: Aspergillus niger

Quick links

Citations

17 citation in other articles

Consistent mutational paths predict eukaryotic thermostability.
van Noort et al. (2013)
16 more

6 mentions without citation

Genomic insights into the fungal lignocellulolytic system of Myceliophthora thermophila.
Karnaouri et al. (2014)
5 more