› 1omi

X-ray diffraction
2.8Å resolution

Crystal structure of PrfA,the transcriptional regulator in Listeria monocytogenes

Released:
DOI: 10.2210/pdb1omi/pdb
PDB entry 1omi coloured by chain, front view.
PDB entry 1omi coloured by chain, side view.
PDB entry 1omi coloured by chain, top view.
Source organism: Listeria monocytogenes
Entry authors: Thirumuruhan R, Rajashankar K, Fedorov AA, Dodatko T, Chance MR, Cossart P, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-149565 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Listeriolysin regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 28.35 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P22262 (Residues: 7-237; Coverage: 98%)
Gene names: lmo0200, prfA
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P212121
Unit cell:
a: 69.43Å b: 72.091Å c: 114.335Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.244 0.278
Expression system: Escherichia coli

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