› 1omv

X-ray diffraction
1.9Å resolution

non-myristoylated bovine recoverin (E85Q mutant) with calcium bound to EF-hand 3

Released:
DOI: 10.2210/pdb1omv/pdb
PDB entry 1omv coloured by chain, front view.
PDB entry 1omv coloured by chain, side view.
PDB entry 1omv coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Impact of N-terminal myristoylation on the Ca2+-dependent conformational transition in recoverin.
Weiergräber OH, Senin II, Philippov PP, Granzin J, Koch KW
J Biol Chem 278 22972-9 (2003)
PMID: 12686556

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-149260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Recoverin Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 23.23 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21457 (Residues: 2-202; Coverage: 100%)
Gene names: RCV1, RCVRN
Sequence domains: EF hand
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I4
Unit cell:
a: 84.333Å b: 84.333Å c: 58.809Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.249
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Neuronal calcium sensor proteins: generating diversity in neuronal Ca2+ signalling.
Burgoyne RD. (2007)
7 more

5 mentions without citation

Double electron-electron resonance probes Ca²⁺-induced conformational changes and dimerization of recoverin.
Myers et al. (2013)
4 more