› 1omy

X-ray diffraction
2Å resolution

Crystal Structure of a Recombinant alpha-insect Toxin BmKaIT1 from the scorpion Buthus martensii Karsch

Released:
DOI: 10.2210/pdb1omy/pdb
PDB entry 1omy coloured by chain, front view.
PDB entry 1omy coloured by chain, side view.
PDB entry 1omy coloured by chain, top view.
Source organism: Mesobuthus martensii
Primary publication:
Crystallization and preliminary crystallographic study of rBmKalphaIT1, a recombinant alpha-insect toxin from the scorpion Buthus martensii Karsch.
Huang Y, Huang Q, Chen H, Tang Y, Miyake H, Kusunoki M
Acta Crystallogr D Biol Crystallogr 59 1635-6 (2003)
PMID: 12925796

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Toxin BmKaIT1 Chain: A
Molecule details ›
Chain: A
Length: 65 amino acids
Theoretical weight: 7.33 KDa
Source organism: Mesobuthus martensii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9GQW3 (Residues: 20-83; Coverage: 97%)
Sequence domains: Scorpion toxin-like domain
Structure domains: Knottin, scorpion toxin-like

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P212121
Unit cell:
a: 30.241Å b: 36.507Å c: 57.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Crystal structure of a highly acidic neurotoxin from scorpion Buthus tamulus at 2.2A resolution reveals novel structural features.
Sharma et al. (2006)

1 mention without citation

Modular organization of α-toxins from scorpion venom mirrors domain structure of their targets, sodium channels.
Chugunov et al. (2013)