› 1onf

X-ray diffraction
2.6Å resolution

Crystal structure of Plasmodium falciparum Glutathione reductase

Released:
DOI: 10.2210/pdb1onf/pdb
PDB entry 1onf coloured by chain, front view.
PDB entry 1onf coloured by chain, side view.
PDB entry 1onf coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Glutathione reductase of the malarial parasite Plasmodium falciparum: crystal structure and inhibitor development.
Sarma GN, Savvides SN, Becker K, Schirmer M, Schirmer RH, Karplus PA
J Mol Biol 328 893-907 (2003)
PMID: 12729762

Function and Biology Details

Reaction catalysed: 
2 glutathione + NADP(+) = glutathione disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188192 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione reductase Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 56.64 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94655 (Residues: 1-500; Coverage: 100%)
Gene names: GR, GR2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P4122
Unit cell:
a: 90.64Å b: 90.64Å c: 123.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.256 0.297
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes.
Deponte M. (2013)
19 more

7 mentions without citation

Heterologous expression of plasmodial proteins for structural studies and functional annotation.
Birkholtz et al. (2008)
6 more