› 1ooe

X-ray diffraction
1.65Å resolution

Structural Genomics of Caenorhabditis elegans : Dihydropteridine reductase

Released:
DOI: 10.2210/pdb1ooe/pdb
PDB entry 1ooe coloured by chain, front view.
PDB entry 1ooe coloured by chain, side view.
PDB entry 1ooe coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Structural genomics of Caenorhabditis elegans: structure of dihydropteridine reductase.
Symersky J, Li S, Carson M, Luo D, Luan CH, Luo M
Proteins 53 944-6 (2003)
PMID: 14635135

Function and Biology Details

Reaction catalysed: 
A 5,6,7,8-tetrahydropteridine + NAD(P)(+) = a 6,7-dihydropteridine + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195045 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydropteridine reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 236 amino acids
Theoretical weight: 24.73 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9XVJ3 (Residues: 1-236; Coverage: 100%)
Gene names: CELE_T03F6.1, T03F6.1, qdpr-1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 41.926Å b: 50.892Å c: 58.77Å
α: 89.98° β: 71.98° γ: 82.05°
R-values:
R R work R free
0.201 0.194 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Structural insights into the dual substrate specificities of mammalian and Dictyostelium dihydropteridine reductases toward two stereoisomers of quinonoid dihydrobiopterin.
Chen et al. (2011)

1 mention without citation

Interaction signatures stabilizing the NAD(P)-binding Rossmann fold: a structure network approach.
Bhattacharyya et al. (2012)