› 1opd

X-ray diffraction
1.5Å resolution

HISTIDINE-CONTAINING PROTEIN (HPR), MUTANT WITH SER 46 REPLACED BY ASP (S46D)

Released:
DOI: 10.2210/pdb1opd/pdb
PDB entry 1opd coloured by chain, front view.
PDB entry 1opd coloured by chain, side view.
PDB entry 1opd coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mutation of serine-46 to aspartate in the histidine-containing protein of Escherichia coli mimics the inactivation by phosphorylation of serine-46 in HPrs from gram-positive bacteria.
Napper S, Anderson JW, Georges F, Quail JW, Delbaere LT, Waygood EB
Biochemistry 35 11260-7 (1996)
PMID: 8784179

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141942 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphocarrier protein HPr Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 9.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA04 (Residues: 1-85; Coverage: 100%)
Gene names: JW2408, b2415, hpr, ptsH
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P1
Unit cell:
a: 25.85Å b: 27.33Å c: 27.6Å
α: 66.9° β: 75.71° γ: 70°
R-values:
R R work R free
0.182 0.182 0.234
Expression system: Escherichia coli

Quick links

Citations

2 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
1 more

22 mentions without citation

A graph-theory algorithm for rapid protein side-chain prediction.
Canutescu et al. (2003)
21 more