› 1oqq

X-ray diffraction
1.47Å resolution

Crystal structure of C73S/C85S mutant of putidaredoxin, a [2Fe-2S] ferredoxin from Pseudomonas putida, at 1.47A resolution

Released:
DOI: 10.2210/pdb1oqq/pdb
PDB entry 1oqq coloured by chain, front view.
PDB entry 1oqq coloured by chain, side view.
PDB entry 1oqq coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Crystal structure of putidaredoxin, the [2Fe-2S] component of the P450cam monooxygenase system from Pseudomonas putida.
Sevrioukova IF, Garcia C, Li H, Bhaskar B, Poulos TL
J Mol Biol 333 377-92 (2003)
PMID: 14529624

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putidaredoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 11.4 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00259 (Residues: 2-107; Coverage: 99%)
Gene name: camB
Sequence domains: 2Fe-2S iron-sulfur cluster binding domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2221
Unit cell:
a: 50.212Å b: 75.845Å c: 107.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.211 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Variations on a (t)heme--novel mechanisms, redox partners and catalytic functions in the cytochrome P450 superfamily.
Munro et al. (2007)
5 more

3 mentions without citation

Crystal structure of the putidaredoxin reductase x putidaredoxin electron transfer complex.
Sevrioukova et al. (2010)
2 more