› 1ou0

X-ray diffraction
2.1Å resolution

precorrin-8X methylmutase related protein

Released:
DOI: 10.2210/pdb1ou0/pdb
PDB entry 1ou0 coloured by chain, front view.
PDB entry 1ou0 coloured by chain, side view.
PDB entry 1ou0 coloured by chain, top view.
Source organism: Thermoplasma acidophilum DSM 1728
Primary publication:
Crystal structure of a predicted precorrin-8x methylmutase from Thermoplasma acidophilum.
Cuff ME, Miller DJ, Korolev S, Xu X, Anderson WF, Edwards A, Joachimiak A, Savchenko A
Proteins 58 751-4 (2005)
PMID: 15609338

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cobalamin biosynthesis precorrin-8X methylmutase CobH/CbiC domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 207 amino acids
Theoretical weight: 22.85 KDa
Source organism: Thermoplasma acidophilum DSM 1728
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HKE7 (Residues: 1-207; Coverage: 100%)
Gene name: Ta0654
Sequence domains: Precorrin-8X methylmutase
Structure domains: Cobalamin biosynthesis CobH/CbiC, precorrin-8X methylmutase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 72.45Å b: 86.82Å c: 120.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.235
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Using nanoliter plugs in microfluidics to facilitate and understand protein crystallization.
Zheng et al. (2005)

1 mention without citation

Crystal structure of a predicted precorrin-8x methylmutase from Thermoplasma acidophilum.
Cuff et al. (2005)